Author Correction: Application of interspecific Somatic Cell Nuclear Transfer (iSCNT) in sturgeons and an unexpectedly produced gynogenetic sterlet with homozygous quadruple haploid

I-12: Nuclear Reprogramming in Bovin Somatic Cell Nuclear Transfer

Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...

histone modification of embryonic stem cells produced by somatic cell nuclear transfer and fertilized blastocysts

objective: nuclear transfer-embryonic stem cells (nt-escs) are genetically identical to the donor’s cells; provide a renewable source of tissue for replacement, and therefore, decrease the risk of immune rejection. trichostatin a (tsa) as a histone deacetylase inhibitor (hdaci) plays an important role in the reorganization of the genome and epigenetic changes. in this study, we examined whether...

Cloned ferrets produced by somatic cell nuclear transfer.

Somatic cell nuclear transfer (SCNT) offers great potential for developing better animal models of human disease. The domestic ferret (Mustela putorius furo) is an ideal animal model for influenza infections and potentially other human respiratory diseases such as cystic fibrosis, where mouse models have failed to reproduce the human disease phenotype. Here, we report the successful production ...

Efficiency of Ovine Fibroblast or Cumulus Cells For Somatic Cell Nuclear Transfer in Sheep

Purpose: Despite remarkable progresses have been achieved in the field of somatic cell nuclear transfer (SCNT), there is little information regarding the effect of donor cell type on the efficiency mammalian somatic cell cloning in vitro. This study compared in vitro developmental competency of sheep enucleated oocytes reconstructed with either fibroblast or cumulus cells. Material and methods...

Cloning of Homozygous 1,3-Galactosyltransferase Gene Knock-Out Pigs by Somatic Cell Nuclear Transfer